Alessio Rosario Marte
1702 Accesses, 4 Citations, 395 Altmetric
Abstract
Immunogenetics, as a branch of science, explores the genetic determinants underlying immune phenomena and the mechanisms by which the immune system shapes the unitary configuration of an organism, conferring individual specificity.
The advent of gene transfection techniques has brought about a revolution in molecular biology, facilitating the targeted modification of genetic material.
In this study, I developed a novel approach that combines immunogenetics with gene transfection, exploiting the potential of viruses and allergens as vectors to modulate gene expression. I recombined viral vectors to penetrate somatic cells by carrying within them therapeutic genes that can counteract such pathogenic mutations. These genes are incorporated into the viral genome and allow the resulting viral particles to act as vectors and effectively deliver corrective genetic material to target cells. This innovative technique, called Allergen-Viral Mediated Precision Gene Modification (Elisa-AVePGM), exploits the distinctive properties of viruses and allergens to cause targeted immune-mediated mutations and improve the efficacy of gene transfection, allowing the modification of pathogenic mutations resulting in improvement and remission of associated disease conditions.
Method
Recombinant viral vectors were engineered to carry therapeutic genes capable of counteracting pathogenic mutations. Allergens were conjugated with viral vectors to enhance cellular uptake and transfection efficiency. The immunogenetic gene transfection technique was performed on target cells, and the efficiency and specificity of gene delivery were evaluated.
To execute an Allergen-Viral Mediated Precision Gene Modification approach, I exploited the ability of Lentivirinae virus to deliver genetic material into host cells by isolating its outer envelope’s proteins to force the receptor lock, penetrate the cells and release the genetic material reprogrammed through selective immunomediation inside it, that was integrated into the nucleus of the host cell altering its mutated structure.
Simultaneously, allergens are utilized to enhance cellular uptake and promote transfection efficiency. Due to their inherent immunogenic properties, allergens stimulate immune responses and cellular internalization. By conjugating allergens with viral vectors, I exploit their allergenicity to facilitate the uptake of viral particles by target cells, augmenting gene delivery efficiency.
Upon entry into the host cell, the viral vector released internally reprogrammed therapeutic genes, which integrated into the host cell genome and exerted their effect through episomal maintenance. Integration or episomal expression allowed prolonged expression of the corrective genetic material, attenuating pathogenic mutations and restoring normal cell function.
The combined use of viruses and allergens offers advantages in terms of target cell specificity, enhancing the precision of the technique. By carefully selecting appropriate viral vectors and allergens, the delivery system can be tailored to target specific cell types or tissues affected by pathogenic mutations, reducing off-target effects.
Considering the potential immunogenicity of viral vectors and allergens, optimization efforts focus on vector design, selecting non-immunogenic allergens, and modulating the immune response to achieve a balance between efficient gene delivery and minimal adverse effects.
Coclusion
The Allergen-Viral Mediated Precision Gene Modification (Elisa-AVePGM) technique represents a novel and promising strategy for modifying pathogenic mutations. By leveraging the strengths of allergens and viruses, this approach offers enhanced transfection efficiency, target specificity, and holds potential for therapeutic applications in various genetic disorders. A concrete prospect for all patients with incurable genetic disease who lack other therapeutic options.
This technique is the result of independent work that needs further research and optimization to fully explore the capabilities in precision genetic medicine.